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It looks like this set of analysis tools is set up for analyzing paired-end sequencing data. Is it also possible to analyze long read data (ONT, PacBio), which aren't in paired-end format?
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Hello,
Just a quick query on adapter dectection, there is smrtbell adapter which keep poping up in our dataset, and looking blasting it at ncbi, not only ours.
I would be nice if it was possibl…
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http://schatzlab.cshl.edu/data/skbr3/
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Dear Developers, hello!
There are some projects using PacBio single-molecule long-read sequencing to analyze full-length transcriptome, but the raw data is in a bam type file and has to be changed in…
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My problem is that I am having difficulty obtaining a list of variants using bcftools mpileup and bcftools call with PacBio data. I do not have this problem with data of Illumina paired-end sequencing…
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Hi all,
I am new to the field and I'd like to verify my variant calling pipeline using latest CHM13 as reference. I have the HG002v1.1.fasta here and I got the truth set v1.1.vcf from NIST.
> (ht…
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Hi, thanks in advance for taking the time to answer this question. What are the constraints of using SUPPA with PacBio sequencing data if any? Thanks again
aa9gj updated
2 years ago
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Here are details regarding barcodes into PCR products once general primer pair is determined to be viable
https://d.pr/f/K2we5f
sr320 updated
1 month ago
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The field will be used to specify the sequencing platform applicable for the application.
It will be located next to the field "prep category".
"Platform" needs a drop down list with the following…
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I tried the program on PacBio hifi reads, which were for amplicon sequencing of some plant DNA samples, barcoded on both ends. The reason is that LIMA generated very simple report missing a lot of inf…