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Howdy,
My name is Per and I would first like to thank you for developing Flye!
Anyway, I have been using Flye to assemble an *E. Coli* isolate from ONT data.
I think that Flye is having 2…
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All plasmids are here: https://www.addgene.org/ASAP_Discovery/
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Goldenbraid plasmid data and maps are also available on this google drive
https://drive.google.com/drive/folders/1QIsJKr4SZyUiKJATi9qJE6ns-60s-K6u
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https://www.ncbi.nlm.nih.gov/genome/plasmids/154?
awk -F"\t" '{print $7}' genomes_plasmids.txt -- for genbank $7, for refseq $6
esearch -db nucleotide -query CP007178 | efetch -format fasta > te…
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Hi,
I am using the PolyA tail estimation in plasmids and I get shorter lengths than expected. I have a few questions about how to set up this feature.
The plasmid I sequenced has a very long poly…
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Related to #58, we need to distinguish between a pre-construction plasmid and a post-construction plasmid (e.g., with an insert replacing a dropout section)
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# What we want
Allow SYC to browse the Resources in eLab (such as plasmids, or primers, or dna sequences...).
# How it could be done
1. User selects "eLabFTW" as a source (similar to Addgene/…
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@gokeson Could you provide advice on how the pipeline should handle plasmids? In a test run I detected a 7.5kb plasmid, full length!
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PLSDB currently hosts only bacterial plasmids. WOuld it be within the project's scope to also add plasmids of Archaea?
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Hub plasmids get marked with a black star in the visualisation, and there's a toggle button that removes them, but any hub plasmids found in later iterations are black circles and don't get removed in…