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Hi all and thanks for your work. According to the documentation, "The first step in any Tombo analysis is to re-squiggle (raw signal to reference sequence alignment) raw nanopore reads. This creates a…
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Hello, marcus1487
This is my first methylation analysis with tombo using nanopore sequencing data.
I hope you understand, even if this is a rather vague question.
I used the 'tombo preprocess an…
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I downloaded data from https://www.ebi.ac.uk/ena/data/view/PRJEB13021 and want to compile the pipeline. Since the whole dataset is too large, I extract 20-30 files from ecoli R7 data for training (eco…
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# #Hi, teacher
I'm trying to call DNA modifications by using deepmod.
First I am successful to create virtual environment and install deepmod step by step through following the command lines…
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When I check whether WarpSTR works correctly by running: bash run_test_case.sh , I get this traceback:
ONT Guppy basecalling software version 6.0.6+8a98bbc
config file: /work/softwar…
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Hi,
I'm using nanodisco to analyse methylation profile from bacteria isolates. I installed singularity (version 3.5.3) and nanodisco (v1.0.3), then I tried to import my fast5 files in nanodisco workf…
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1. As an exercise, I found installation using conda on a macbook air (early 2015) with Mojave operating system was fine. The only issue was one flagged after using the plot command on files generated …
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# Issue Report
## Please describe the issue:
Hello, I am currently performing target-specific custom barcode demultiplexing using Direct RNA seq data.
In the options, there is a setting for m…
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Hi，
I am trying to run eventalign on reads from RNA004 and I get the following error:
[meth_main::13.631*0.41] 0 Entries (0.0M bases) loaded
[pthread_processor::13.631*0.41] 0 Entries (0.0M bases…