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Hi all and thanks for your work. According to the documentation, "The first step in any Tombo analysis is to re-squiggle (raw signal to reference sequence alignment) raw nanopore reads. This creates a…
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Hello, marcus1487
This is my first methylation analysis with tombo using nanopore sequencing data.
I hope you understand, even if this is a rather vague question.
I used the 'tombo preprocess an…
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I downloaded data from https://www.ebi.ac.uk/ena/data/view/PRJEB13021 and want to compile the pipeline. Since the whole dataset is too large, I extract 20-30 files from ecoli R7 data for training (eco…
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Hi,
I tried to run f5c eventalign using the merged blow5 file that I created from slow5tools and here is the error I got:
```
[slow5_idx_init::INFO] Index file not found. Creating an index at '…
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When I check whether WarpSTR works correctly by running: bash run_test_case.sh , I get this traceback:
ONT Guppy basecalling software version 6.0.6+8a98bbc
config file: /work/softwar…
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Hi,
I'm using nanodisco to analyse methylation profile from bacteria isolates. I installed singularity (version 3.5.3) and nanodisco (v1.0.3), then I tried to import my fast5 files in nanodisco workf…
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# #Hi, teacher
I'm trying to call DNA modifications by using deepmod.
First I am successful to create virtual environment and install deepmod step by step through following the command lines…
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Hi, developer.
Thank you for providing a useful tool!
I have run xpore and generated the result with HEK293T-WT-rep2 and HEK293T-Mettl3-KO-rep2 successfully.
I wanted to check whether I correctly…
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1. As an exercise, I found installation using conda on a macbook air (early 2015) with Mojave operating system was fine. The only issue was one flagged after using the plot command on files generated …