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Hi,
CCPE is a useful tool to compute cell cycle pseudotime using single-cell RNA-seq data with ground truth. I also found that the paper described that CCPE could be used to analyze datasets without …
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Hello,
I have a single cell dataset with immune cells sorted from placentas. Recently I found out that the immune cells could be either fetal or maternal, even at the beggining of the gestation. Si…
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### Name of the tool
cellranger multi
### Tool homepage
https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov
### Tool description
is a…
pcm32 updated
3 weeks ago
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### Is your feature related to a problem?
The **duplex reads don't contain primers nor adaptors** due to how duplex works, the duplex reads themselves will have the adapters and primers trimmed off. …
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## Purpose
To get consensus on what the Vulcan config should look like.
Context:
Typically a snakemake pipeline is just:
- a Snakefile
- a config
- some scripts.
A vulcan pipeline …
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Hi,
Thanks for developing such an excellent tool for single-cell genomics. I am wondering whether you could provide the processing pipeline for Easysci-ATAC-seq.
Thanks.
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Hi @hxj5 , I have a question regarding 10X 5' scRNA-seq data.
For 5' sequencing, the read containing cell barcode and UMI contains part of the transcript https://kb.10xgenomics.com/hc/en-us/article…
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Dear Developers,
Thanks for introducing such a tool to detect multiplets in single-cell epigenomic data.
I’m completely new to using this tool and have some simple questions. I have successfully…
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Annika and I want to speed up the processing of single-cell multiome datasets.
So I may need to install 10X pipeline on SCG.
To check out what is already there, I have to:
1. Talk to Ramesh
2.…
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I am working on converting an old pipeline that used guppy_basecaller, tombo, and meme to process a methylation experiment.
I have the data in fast5 format. In the past, I used guppy as follows.
`…