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Hey, great tool!
I wonder if it is also possible to use more than one primer pair? The example primer FASTA and the description sound like one can only use one primer pair. But what if I sequenced …
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Dear authors,
Thank you for your work, I was testing your software on several datasets and I obtained this error message:
```
[raven::] loaded 9004 sequences 0.110631s
[raven::Graph::Construct] …
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Hello,
We are using CRISPResso2 pooled to map reads to multiple amplicons and it's working very nicely as long as the guide matches the amplicon perfectly. However, we also have cases where a guid…
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Dear Sirs, I have some problem about tmp file which was not created. I ran the job in linux server. There is some error in below:
passedQC_iF02_iR09.fasta contains 17 reads.
--> Low number of read…
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I have the output of singleM. But I wanted to ask if I can use the genes for example calculate and visualize the individual genes richness, etc or this is meant to use the singleM as a whole without a…
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Hi Saskia!
Hello from Canada!
I am using Decona to genotype some amplicon data and I have run into some trouble.
First, I was able to run the example data with no problem.
But, with, my …
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Hi mate!,
Love your program it has become my default to go for clustering and consensus.
I have been using it with the error below sporadically poping sometimes, but recently it is happening e…
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### Ask away!
I have observed an error with the sequences of the inserts in the FASTA format: {{ alias }}.insert.fasta. I investigated the issue and it appears to originate from the following code …
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**Current term details**
```
Term name - 16S recovery software
Term ID - MIXS:0000066
Structured comment name - x16s_recover_software
Definition - Tools used for 16S rRNA gene extraction
```
…
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Hi, I am trying out Ampligone. It works fast but I got a few questions. I am working with Nanopore reads of 16s rRNA amplicons.
1) If you provide the tool with a single reference, than it determine…