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I got this error while starting to run this pipeline, any ideas how to fix?
AttributeError in line 6 of /home/data/Megan/nanopore/pipeline-transcriptome-de/Snakefile:
'Workflow' object has no attr…
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Hello,Professor
I have `HiFi + HiC, Nanopore + HiC data`
The draft assembly of `HiFi data` was finished by `Hifiasm`, draft assembly of` Nanopore data` was finished by `Nextdenova` and the resulti…
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Greetings,
Thank you so much for this amazing tool!
So I am checking the consistency between the SV calls after randomizing and splitting the reads into two equal datasets. Each dataset is of arou…
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Do you think centrifuge would be successful at classifying 16S reads from Oxford Nanopore? The nanopore reads cover the entire 16S gene (1522bp) but have a high error rate (10-15%). Have you tried …
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Dear,
I am writing to get input from experienced ones. I got nanopore data and using q2ONT command line to process my 16srRNA gene seq data. After demuliplexing, adapters removal, and trimming the re…
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Depending on what data is available at any moment, we may need to perform analysis with only short (illumina) reads, only long (Oxford Nanopore aka ONT) reads, or a hybrid analysis using both short an…
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ONT data does needs adapter chopping, quality filtering and length filtering. Adapter chopping for nanopore now references porechop, which is unmaintained as of 2018. Nanoflt is also recommended. I th…
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Hello MerenLab,
While at EBAME I was encouraged to open this discussion by @FlorianTrigodet and @ivagljiva, and bring it to @semiller10's attention.
DNA methylation is on the verge of constituti…
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It looks like this set of analysis tools is set up for analyzing paired-end sequencing data. Is it also possible to analyze long read data (ONT, PacBio), which aren't in paired-end format?
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Can anyone tell me why I am getting this error and how to fix it?
`/projects/nanopore-working/niki/bin/anaconda3/lib/python3.6/importlib/_bootstrap.py:219: RuntimeWarning: This module has been dep…