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### Operating System
CentOS 7
### Other Linux
_No response_
### Workflow Version
v1.8.3
### Workflow Execution
Command line
### EPI2ME Version
_No response_
### CLI command run
NXF_SINGULAR…
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### **Description**
I wish to use the latest NanoSim v3.0.1 (supports reading .gz sequence files, and bam files), but `read_analysis.py` does not complete. The `primary.bam` file is not indexed, an t…
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Hi there, I am trying to do genome assembly using several tools and the below script:
```groovy
#!/usr/bin/env nextflow
nextflow.enable.dsl=2
/*
*
=====================================…
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### Ask away!
Hello,
I downloaded human Nanopore data from NCBI. This MinION data is 79.72 Gb.
https://www.ncbi.nlm.nih.gov/sra?linkname=bioproject_sra_all&from_uid=553438
I searched this we…
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### Operating System
Ubuntu 22.04
### Other Linux
_No response_
### Workflow Version
0.3.31
### Workflow Execution
Command line
### EPI2ME Version
_No response_
### CLI c…
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I see in previous issues that you currently recommend the WGS mode to analyse Nanopore data using crispresso2.
May I ask why?
Thank you for the hard work!
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Hi,
I have been using bambu lately for novel isoform identification from long-read RNA-seq data these days. And it is efficient and useful.
However, an issue happened when performing novel isoform…
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This workflow can work for sample.fastq.gz using zymo database, however, Not work in hpvc database. I wanna know which cause this error. Thanks!
It can work as follows:
```bash
OUTPUT=output
n…
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### What happened?
Just trying out the workflow on my local machine with the included test data. Works fine without the approx_size_sheet.txt, but when I _do_ specify it, the workflow does not perfo…
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Hi,
I'm wondering that the input file(s) are for calculating and plotting the empirical error distribution? I'm very interested in being able to access that part of the pipeline as a standalone comma…