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Hi,
many thanks for developing and maintaining this very cool tool! We could already obtain some very nice insights using sciCSR! However, as you pointed out in your tutorial using SHM as mode for …
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Hi, I wonder if there is any function in celldancer which can generate the raw RNA velocity matrix. I intend to compare this method with other RNA velocity estimation method. Thanks a lot.
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Hi,
Thanks for the nice work and code!! For the datasets (S6-S17) jhpce#HumanPilot10x, how could I get the RNA velocity? I'm trying to use the velocyto package to generate .loom file. I'm wonderin…
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Hi,
I was wondering whether RNA velocity works well for 5' 10x data and if the results are similar to 3' data?
Many thanks,
Lucy
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Hi there,
I was trying to write my adata with `write_h5ad` which has my loom objects merged with my Seurat object in Python so that I can then convert it to Seurat object to use `SCP`, but it thre…
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Hello. I used velocyto to generate the loom file required to RNA Velocity using seurat wrappers. I am unable to convert the loom object to Seurat as shown in the tutorial. I get the following error. A…
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https://mp.weixin.qq.com/s/QIbmIE7Gs-4qxUbizjwC8w
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Hello CellDancer Developers,
I'm working with multiple single-cell sequencing samples and am seeking advice on the best approach to analyze RNA velocity using CellDancer. Should I merge the samples…
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Hi PAGA community,
In the PAGA genome bio paper fig3 c,d, it shows how users could integrate RNA velocity information with PAGA to generated a directed PAGA graph. I really think that this is an am…
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I am trying to analyse RNA-velocity for single-nuclei RNA sequencing data from 10X platform. I am not sure in detail how it calculates the unspliced/spliced rate when I run the command 'velocyto run10…