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I have a 7x WGS ONP genome and my jobs crashed after 64hours. Is this normal? I started with raw FASTQs and using default parameters except for the nanopore option.
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Hey all,
I recently started using Nanopore sequencing and after the assembly steps I have a problem when running nanopolish.
First I assembled the genome using Canu. To do so, I used the basecal…
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Hi, I'm using pychopper for a test data generated with the PCR cDNA kit for minion. Pychopper (with the default params) finds only 25% of the reads to be "full length cDNA reads". The primers are the …
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Running ```./canu/Linux-amd64/bin/canu -p metagenome -d /scratch/metagenome_folder genomeSize=1g -nanopore-raw metagenome_folder/metagenome.fastq```, I'm getting a Error 7 as follows:
```-- Startin…
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Hi Mikhail,
Flye is working great for my mammalian genome assemblies (using raw Nanopore data). One of the species I'm working on has an existing reference completed over a decade ago with Sanger dat…
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Hi Hyeshik,
I was trying to execute the command as below and there was error.
```
poreplex -i ./fast5 -o ./output --trim-adapter --barcoding --keep-unsplit --fast5
Poreplex version 0.1 by H…
EPKok updated
6 years ago
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I'm trying to understand what I can do about these errors:
```
$ tombo resquiggle /Volumes/Macintosh\ HD/Library/MinKNOW/data/reads/Reg_RNA_copy/fast5/pass/0/ /Volumes/Macintosh\ HD/Library/…
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Hi Luigi,
I run the docker and meet this error:
``` ###RUNNING iASSEMBLER 01:32:04 17-07 ###
Traceback (most recent call last):
File "/opt/LoReAn/code/lorean.py", line 538, in
main()
Fi…
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Hello again,
In Dip-C paper, there is a cross section view of the diploid interphase mESC cell 1CDX1-413 and some other cells. The model and its analysis looks good.
In the paper by Nagano et al.…
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Hello,
I want to use ailgnQC to analyze some nanopore RNA data, but I keep getting this allocation error:
alignqc analyze aln.bam -g ../Mus_musculus.GRCm38.cdna.all.fa -t ../UCSC_Main_on_Mouse__…