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Hello, I am trying to align metagenomic sequences, when I run initPipeline with the following command I get the expected output:
`initPipeline -q -1 Unmapped.out.mate1 -d projectDir -i 300:800`
Pr…
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## The need
To annotate pan-genome genes, the user is encouraged to export from an existing pan-genome database via `anvi-get-sequences-for-gene-calls -g` and then annotate the genes via their own …
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Hi, I ran PASA with singularity to perform transcripts assembly and annotation update. The alignment assembly step ran successfully, but the update step ran with error, here are the error information:…
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I think it would be useful to have a module for this recently published genome assembler [hifiasm](https://bioconda.github.io/recipes/hifiasm/README.html)
- [x] This module does not exist yet with …
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Dear developers,
Thanks for your great software!
I am working on assembling a putative hexaploid or octoploid plant genome with a genome size of ~1.45 Gb by flow cytometry. In addition, the only…
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Hello I want to build my own database (GTDB + own built MAGs). I used prodigal to convert my nucleotide fasta files to protein fasta files.
As I see prodigal assigns as first column of a fasta heade…
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Hi,
I am trying to assemble three plant mitochondrial genome sequences using Novoplasty. I successfully assembled two mitochondrial genomes. But When I try to assemble the third genome sequence usi…
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## **Overview**
From internal testing, [wtdbg2](https://github.com/ruanjue/wtdbg2) (long read SV assembler) performs well for assembly of breakpoints, something that MAVIS must implement.
The fol…
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Dear developers of this great assembler,
I have a question concerning a weird k-mers histogram with 3 peaks for a definitely diploid fish genome. Please find the attached k-mers histogram as output…
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Hi Ryan,
I'm trying to finish some mitochondrial genomes that have initially been assembled from PacBio reads with Canu. The Canu assemblies give me a single contig, but almost always fail to circu…