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Dear Will,
I currently work with a dataset containing both gene expression data and surface marker information, using cite-seq. Now I wonder if you think there is any inherent problem with using all…
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Hi,
Thank you very much for your meaningful work.
I am a student from sun yet-san university. Recently, I have been doing some work about bivalent genes during the development process. I am…
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In the Li 2018, [Cell](10.1016/j.cell.2018.11.043) publication they find a continuum between cytotoxic cells and cell expression exhaustion markers. They also show that cells expression markers of wha…
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hi,
I download the vOTU.fna (There are 5251 vOTUs in there.)data in the text and used the MGV pipeline(https://github.com/snayfach/MGV/tree/master/viral_detection_pipeline), like this
````
cond…
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Hi,
Dose anyone encounter the same error when run the gene-based analysis using SAIGE-GENE?
In my first try, I used --minMAC=0.05, later I found that minMAC=1 can fix the issues for some regions …
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Joana provides a good description with implementation ideas:
Here’s what I am thinking:
We want to cluster based on the gene expression and then color the cells based on the sequence barcode for…
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Hi there,
I used DWLS to predict the composition and failed to identify T cells in the Visium data. The single-cell dataset I used comprises enough cell types that could exist in the Visium samples…
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Hi,
I have installed singlem with conda, and running the below command:
`singlem pipe --forward /home/ps897/sharing/pmb_study/fastq_files/SQ8353_1.fastq.gz --reverse /home/ps897/sharing/pmb_study/…
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Hello,
I was initially drawn to this package because in the original publication, it was said that we could use it to deconvolve bulk RNA-seq data and assign cell types using marker genes, but appa…
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https://doi.org/10.1016/j.cell.2012.08.023
# Authors
Buganim, Y., Faddah, D.A., Cheng, A.W., Itskovich, E., Markoulaki, S., Ganz, K., Klemm, S.L., van Oudenaarden, A., Jaenisch, R.
# Year
…