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### Ask away!
Hi all,
We are working with Minimap2 within the EPI2me lab platform and trying to build a custom database based on the 1,500 full-length 16S rRNA sequences present in the [HOMD](https:…
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There are two common ways to sequence a DNA: short-read and long-read sequencing. As of right now, YEAT only supports short-read sequencing. Adding long-read assembly support will be a tremendous addi…
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Hi I am running wtdbg2 on my viral metagenomics shotgun fastq files 56k reads. I was able to assemble long reads, but was unable to create a consensus ctg.fa file. What do you recommend running for …
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Results have been [aggregated](https://nbviewer.jupyter.org/github/bebatut/galaxy-training-m…
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I have downloaded and creatd kraken database successfully..but when I run krakenunique to classify my sequence it shows error saying "krakenuniq: database ("/home/mcs/database/krakenunique/db") does n…
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Hi! I am trying to run the pipeline for 12 samples, with the following parameters:
`nextflow run EBI-Metagenomics/emg-viral-pipeline --fasta "./contigs_rnaSpades/*.fasta" --cores 8 -profile local,d…
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I'm trying to build the database for my metagenomics analysis, and I've run the following commands but the database is taking too much disk space (bacteria was already 35gb and only 19% was downloaded…
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I've been running kraken2_server/client within a nextflow pipeline and am repeatedly seeing the following error:
```
Connecting to server: localhost:8080.
Classifying sequence stream.
Server respo…
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We are close to releasing our new pipeline schema which describe input parameters. Through chatting to people at BOSC 🍐 I've become aware of a project called [Bioschemas](https://bioschemas.org) which…
ewels updated
3 years ago
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Hi!
I am trying to run VIRify in an HPC cluster using nextflow (22.10.4.5836), slurm and singularity (3.4.2).
This is the command in my sbatch script
`nextflow run /CCAS/home/cpavloudi/.nextf…