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Hello Kristoffer,
I ran `NGSpeciesID` with the following command:
```
for file in *.fastq; do
NGSpeciesID --t 4 --ont --fastq $file \
--consensus --medaka \
--abundance_ratio 0.005 \
--rc…
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Hi there,
Have been using your library to demultiplex libraries - 384 well illumina libraries per ONT run - which have a similar construction to Tru-seq paired indexes.
The first sets tested w…
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Somewhat an extension of #75, but there have been improvements to the `uchime_denovo` algorithm of vsearch. Specifically:
- uchime2_denovo
- uchime3_denovo
As outlined in the [vsearch manual](h…
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I have the output of singleM. But I wanted to ask if I can use the genes for example calculate and visualize the individual genes richness, etc or this is meant to use the singleM as a whole without a…
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Hi,
I am really struggling with convector. I try to launch pipeline.py but results me in some errors so I tried a step by step procedure. I have a folder of BAM files and a .bed file correctly format…
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Make a decision on how to use the data from overlapping amplicons.
Right now only one is used, which is an issue if they have conflicting information, and also if only 1 pool is used and the marker …
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I am struggling to completely understand the differences of the outputs in AA and AC and how to translate this to presenting the results of the analysis:
If I have an individual sample which appears …
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Hello,
I like dadasnake and I am using it for processing 16S rRNA amplicons. I have some data from ITS and saw there is support for ITSx. However, there is an newer alternative for ITSx that uses f…
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Primer erosion with omicron is showing itself to be fairly important. In some cases, masking problematic amplicons may help improve the interpretability of the data. Is it worth bringing that out here…
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**hi,thanks for your great software!
When I plan to use full mode to design primers and check primer specificity, or check primer specificity, the progress bar of the task will run all the time. I …