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Hi,
I have setup a playback run using MinKNOW v5.8.13, minION MK1B, selected all default options and using readfish for basecalling. I can't figure out from the documentation where is the output. …
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### Is there an existing issue for this?
- [X] I have searched the existing issues
### Have you loaded the SQANTI3.env conda environment?
- [X] I have loaded the SQANTI3.env conda environment
### …
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### Ask away!
Hello,
I'm working on aligning cDNA RNA-seq ONT data. I noticed that the "--minimap_preset rna" parameter is designed for Nanopore Direct RNA-seq. Do I need to adjust the code…
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Dear @cjw85 ,
Since 2022, our laboratory has been utilizing Oxford Nanopore Technologies (ONT) sequencing for bacterial isolate sequencing. Last year, ONT has introduced Dorado as a new basecaller,…
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Hi,
We really would like to test and use your program, but we encounter some problems with installing it. It seems like the same problem that 2tony2 mentioned. So we tried his suggestion and instal…
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### Ask away!
Hi all,
I wrote my script but when I launch it it crashes without any error.
This is the command I run:
```
REFGEN=/data/references/genomics/hs37d5.decoy.fa
REFTRAN=/data2/refe…
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Hi!
I am ghaving an error that I do not understand from deeplexicon.py. The output is empty.
Could you please let me know what's going on and how to solve this issue?
Thank you!
```
[...]
F…
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I recently tried your workflow, and found mosdepth was crashing due to a dependency issue. I was able to fix it by providing the versions that you have listed on the Structural variation analysis page…
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Hi,
I am trying to run eventalign on reads from RNA004 and I get the following error:
[slow5_idx_init::INFO][1;34m Index file not found. Creating an index at '/path_to_/sample.blow5.idx'.[0m
[s…
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# Feature being added - Filtering!
Filter reads and alignments based on a flexible "mini language" specified in the TOML.
Would go into config TOML under the respective place for the filtering? I.e…