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Hello,
I apologise if this is not the right location to make this type of enquiry. I am completing my master's in diagnostics genomics and I have some concerns and questions that I wanted to reach …
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Hi
Could you please clarify who "simplex reads which have duplex offsprings" are and their utilities? Apology if this has been asked before but cant find a resource that explain about it.
Looki…
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Hi,
It seems that this event detection is similar to scrappie, I'm a little bit confused about these parameters. It seems that the length of event is much longer than basecalled nucleotide reads?
T…
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Hello, I hope you are well!
I'm able to download a specific NCBI Assembly via `bactopia --accession [accession]` -- is there a way to do so with a list of NCBI Assembly accessions (i.e bactopia --…
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Hello, I'm trying the newer version and getting the following error with a fasta of 5 ONT reads:
```
Shasta Release 0.12.0
2024-May-13 11:33:45.266965 Assembly begins.
Command line:
shasta --i…
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### Description of the bug
I have Nanopore reads aligned to the GRCh38_hmf reference using minimap2, as it's the recommended tool to use for Nanopore reads. I get an error message at the process NF…
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Describe the issue:
Hello, i am trying to recreate the steps done in this post: https://google.github.io/deepvariant/posts/2021-02-08-the-haplotype-channel/
I have a bam file with phasing informatio…
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I'm using wtdbg2 to perform by phase block local assembly for the human genome using Nanopore reads(48x). I used the phase block length as the estimated genome size. However, the final fasta file size…
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I'm a researcher working with **nanopore** sequencing data, and I have a question regarding the various numeric values reported in the NanoPlot output.
Following is the the **NanoPlot** report for …
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Hello, thank you for this package.
I have 3 questions and I hope you can answer them:
- Can we use Variant Detective on reads generated using Oxford Nanopore technology (long reads)?
…