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I am assembling tilling amplicon data with canu, the amplicon length varies from 500 to 1200 bp. With canu, I can assembly some of the region together to a single contig but there are some other regio…
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Dear Sirs, I have some problem about tmp file which was not created. I ran the job in linux server. There is some error in below:
passedQC_iF02_iR09.fasta contains 17 reads.
--> Low number of read…
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Hi Saskia!
Hello from Canada!
I am using Decona to genotype some amplicon data and I have run into some trouble.
First, I was able to run the example data with no problem.
But, with, my …
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Hi mate!,
Love your program it has become my default to go for clustering and consensus.
I have been using it with the error below sporadically poping sometimes, but recently it is happening e…
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Hi, I am trying out Ampligone. It works fast but I got a few questions. I am working with Nanopore reads of 16s rRNA amplicons.
1) If you provide the tool with a single reference, than it determine…
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**Current term details**
```
Term name - 16S recovery software
Term ID - MIXS:0000066
Structured comment name - x16s_recover_software
Definition - Tools used for 16S rRNA gene extraction
```
…
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Hello.
I'm checking the files for download and I see some file with name "refseq207nr_classifierpairA.qza", are this files for use with qiime2? Why are there classifierpairB and C?
What is the c…
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Hi, I am testing crabs to get a reference library of 12S. I noticed that after step 4 I got some small size sequences (for example 20 bps) and in other cases some sequences that bind only by one of th…
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Hi,
I have analysed ITS1 amplicons from fungal samples (leaf endophytes) using kraken2 and the PlusPFP-16 indices provided by Ben Langmead.
These amplicons were trimmed for PCR primer sequences, b…
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I'm trying to cluster with VSEARCH and assign taxonomy to a set of samples using partial LSU amplicons (in fasta format) PREVIOUSLY extracted from ONT long reads using ITSx. I used the following param…