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Hello,
Unicycler has been repeatedly crashing while running a hybrid assembly with Illumina reads + GridIon long reads.
The error is (in more than 3 attempts):
/software/pathogen/internal/prod/bin…
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Hi!
I am grying out this tool now. I installed via conda, and am using it on a slurm run cluster with srun. Have asked for 10 cpus. I have a file list containing 8 plasmid fasta seqs. The plasmids …
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Hi there
Hybrid assembly gives following error:
Using CABOG from is MaSuRCA-3.2.6/bin/../CA8/Linux-amd64/bin
Running mega-reads correction/assembly
Using mer size 15 for mapping, B=15, d=0.02
…
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Add another hybrid strategy for samples where this might be the best option.
This strategy is to perform a short reads assembly and then scaffold with long reads.
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hi
I noticed a strange behavior of MaSurCa for hybrid assembly. I have 10 samples (they are all different for sure), each sequenced using both Illumina and MinION and i made hybrid assembly using M…
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Hi, I'm trying to do _de novo_ assembly of a unicellular eukaryote genome that has an average size of 114 Mbp. I've successfully run Masurca 3.3.5 with pacbio `+` Illumina pair reads. Afterward, I wan…
zeyak updated
4 years ago
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**Minecraft Version :** 1.20.1
**Mohist Version :** Build 808
**Operating System :** Windows 11
**Concerned mod / plugin** : Worldedit Plugin/NoMod and FAWE are incompat…
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Hello,
I have an hybrid scaffolding assembly (hifiasm assembly with hifi Pacbio reads + Bionano optical map). I would like to know if it is suitable to run purge_dups with this kind of assembly (wh…
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Depending on what data is available at any moment, we may need to perform analysis with only short (illumina) reads, only long (Oxford Nanopore aka ONT) reads, or a hybrid analysis using both short an…
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Hi,
I'm trying to run masurca with a hybrid assembly of illumina PE and Nanopore data and I get the following error after running the config file:
Error line 23 of configuration file 'sr_config…