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Hi, I'd like to use openmmtools's replica exchange code, but I can't see a way of actually demultiplexing the trajectories and having a look at them. Am I missing something? The docs don't address thi…
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Hi,
I was wondering if I could use this method to help me quantify my Nanopore library with barcode sequence?
I have already demultiplexed the ONT library to each individual barcode by using some o…
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Hi there, I was wondering if there is a way STARsolo can handle demultiplexing sample tags. I have 3 samples that have undergone cell hashing, where they have oligo-tagged antibodies then pooled into …
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I'm trying to demultiplex illumina paired end 2*250 data. I have a single forward fastq file and a single reverse fastq file and a mapping file, but no index reads file. I tried creating an index r…
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Please add the following instructions to somewhere:
For FASTQ output from buttery-eel, we call guppy_barcoder which comes with the ONT Guppy package for demuxing. It seems that guppy_barcoder does…
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Hi there,
Is there a way to demultiplex simultaneously while basecalling for mod bases using Megadolon?
:-)
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For some workflows, you may already have unmapped BAMs when you're ready to use splitcode and it would be helpful if you weren't required to convert them back to FASTQs.
As a specific example, PacB…
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Dear, I am new to nanopore community and have some questions. I might sound silly!
We preapred nanopore librariy using 1-24 SQK-16S024 kit for 24 samples. After sequencing, i got fastq files, they we…
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**Addition Description**
It would be useful to bin reads by primer prior to primer removal. I'd like to separate a single FASTQ-based artifact (containing several different primers) into multiple out…
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Hi,
I am very excited that there finally is a tool out that does proper UMI quantification! I will retire my [heuristic method](https://github.com/vals/umis) and recommend this in it's place.
There…