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Original Author: Eli Fisker
Original Link: https://getsatisfaction.com/eternagame/topics/-strategy-market-switch-jl-s-microrna-fish-hook-1
Intro
This strategy is courtesy to jandersonlee and based …
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The current delta-cq vignette shows how different forms of qPCR data can be incorporated and analysed by tidyqpcr but is not a good example of plate design according to the tidyqpcr ethos. A new vigne…
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Hi,
thank you for developing a good package.
I have the strange question. Can i adapt this package for RNA-seq data or it can be used only for single-cell?
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Episode 1 does not cover what is listed in the questions, particularly "What are the different choices to consider when planning an RNA-seq experiment?". This episode needs to be greatly expanded to i…
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An interesting point was raised by Aya: how can we be sure the plasmid contained in the mother cell will be transferred to the minicell?
It was then suggested that we look at the iGEM Vilnius-Lithu…
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Hello,
Question about 'RNA sequencing assay' (OBI:0001177) and 'RNA-seq assay' (OBI:0001271). In OBI, 'RNA-seq assay' is a subclass of 'RNA sequencing assay'. Are there examples of assays that fal…
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Hi,
How do I access the the variance of ambient rna calculated for each cluster? Or, is the parameter p used for estimating ambient rna the same across all clusters?
I have run the souporcell p…
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Hi,
I was wondering. What is the best way to incorporate biological replicates in RASQUAL study design? I have RNA-Seq/ATAC-Seq data for 100 individuals (2 biological replicates per individual)
The …
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Hi
Great work on the R-package Augur!
I'm using it to prioritise cell types response on treatment on a disease in two setups: 1x treatment and 5x treatment, and I have a couple of question on ho…
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Awhile ago I made a biopython issue mentioning a small bug I saw in the DNA nearest neighbor values. I caught that because I was using biopython's DNA nearest neighbor energies to bootstrap a library …