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Hello, I am running Lotus2 on merged paired end illumina reads and am coming up with a weird issue. I am able to go through read filtering then I get an error tha the SDM file is not empty, but when i…
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Hi @clintval,
do you have any plans of supporting the new SampleSheet v2 format?
Seems Illumina has released new tools and along a new version of the Samplesheet.
(https://blog.software.illumin…
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Hi,
I have a particular case and wanted to know If I can address this with `minimap2`. I have some Illumina reads that come from several amplicons and I also have a list of primers that generated …
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The workflow requires paired-end data, but we may be able to use just the first read (when the Illumina adapter are ligated on the PCR amplicon product in random orientation).
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Hi Sai:
Thanks for sharing these scripts.
I did some tests with the SHARE-seq technique, which selected the illuminat 300cycles sequencing strategy, and everything else was bas…
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There are quite a few duplicate positions for markers with differrent names in the raw file set FinalReport_54kV2_collection_ed1.ped and FinalReport_54kV2_collection_ed1.map.
Found these:
```
CH…
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Hello I am just starting to experiment with the PyPiRet tools (at CDC).
Does the pipeline work with single ended/unpaired illumina data ?
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Hello,
I ran "!pip install git+https://github.com/Illumina/Cyrius.git" in a cell in my jupyter notebook and received the following output:
Collecting git+https://github.com/Illumina/Cyrius.git
…
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I use the following command to assembly my illumina data with nanopore, but there are a warning showing below.
My command:
nohup ~/tools/SPAdes-3.13.0-Linux/bin/metaspades.py -1 ~/data/seameta_ill…
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### Description of the bug
Hello, I am running the test command to launch the pipeline on an HPC machine, but with version 2.6 I am having issues as it fails at the NFCORE_VIRALRECON:ILLUMINA:VARIANT…