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Hi
From #14 It seems that MotifSeq could demultiplex a bundle of fast5 to retrieve the fast5 for each strains. Is this right? Or there is some options in Squiggle Kit to do the same?
Thanks
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Hello, I have been encountering a recurring error while performing call-methylation:
`'entries: 43715, qc fail: 0, could not calibrate: 0, no alignment: 43715, bad reads: 43710.'`
The analysis p…
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Hi,
Everything runs very well and no error message is given. However, the final result files are just missing. I tried it twice on an Arabidopsis thaliana data set.
PacBio reads from this study:…
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I performed buttery-eel basecalling on the slow5 file and got the fastq file. I want to detect m6A modification. Can I extract information from the slow5 file and the resulting fastq file to build a m…
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### What container were you trying to use, and how were you attempting to use it?
Is anybody know why the tool doesn't work when I give in input the diploid genome?
The diploid genome is generated …
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Can this pipeline also demultiplex reads from cell barcodes?
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Hello,
Executed code:
shapemapper --name example --target ../FASTA/RNA.fasta --out RNA_shapemap --amplicon --modified --R1 100mM_1M7_RNA_R1.fastq --R2 100mM_1M7_RNA_R2.fastq --untreated --R1 DM…
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Hello,
I have a question: according to Oxford nanopore their last cells produce very accurate reads. Does "map-ont" still work as the best setting to map those reads? I am asking because the manual…
ghost updated
1 month ago
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Hello,
I'm using racon to polish a close-to-complete genome assembly where most contigs have telomeres on the end. Racon is removing many of these telomeres. Is there any way I can avoid racon remo…
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@jrobinso @mschatz @marcus1487
Split from #1133. Related to #1153.
This issue splits from #1133, which provides nice support for visualizing 5mC methylation (C+m in MM tag), to support other ma…