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Good day, colleagues,
I have a question regarding the search for several RNA modifications, including:
- N6-methyladenosine (m6A)
- pseudouridine (Y)
- 5-methylcytosine (m5C)
- 5-hydroxymeth…
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We are using the scripts which you published on this paper and repeat the m6A calling using EpiNano, with the datasets published on Nature Communication (2019) including Curlcakes and yeast datasets.
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TTT16 updated
2 months ago
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I am trying to obtain m6a modifications from direct RNA sequencing of the avian influenza virus. It seems I have been able to obtain the eventalign.txt file... but after running m6anet dataprep the o…
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Are you adding any features for CRISPR-Csm transcript targeting systems?
https://www.nature.com/articles/s41587-022-01649-9
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Hi! Appreciate the great work.
Is there a way to predict the structure of m6A modified RNA through this tool? I tried the web interface and I can't seem to find a way to incorporate m6A in the FASTA …
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I have RNA modbams with m6A (in DRACH motifs) and everything runs without error but wgmeth/differential methylation results are sort of confusing - are there going to be RNA-specific additions to meth…
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Hi!
Thanks for your nanocompore to detect direct RNA modification. But I have some question about the output file.
I got the normal output like:
```
pos chr genomicPos ref_id strand ref_kmer GMM_l…
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I have direct-RNA sample nanopore data one is control and another is treated sample. So I just want to know the m6A modification in my treated sample. I used human_transcript.fasta file for resquiggle…
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Hi @hasindu2008 ,
Thank you for developing f5c !
I wonder know whether f5c eventalign can output basecalling kmer?
Generally, f5c eventalign will output the following header files.
contig posi…
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Hi, I encountered an issue when using buttery-eel to baseclling with dorado-server.
![image](https://github.com/Psy-Fer/buttery-eel/assets/87375686/b0cd2c9b-f4ba-4ed0-aa7a-f2efe763199d)
I want to us…