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Hello!
I'm trying to run a bulk RNA-seq analysis using the following template:
```
# Template for human RNA-seq using Illumina prepared samples
---
details:
- analysis: RNA-seq
genome…
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thank you so much for this great work!!!
could you please provide more train datasets about ovarian cancer ?
i am so pleased that you would reply to this messages
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**tl;dr**: 3-tag sequencing methods for bulk RNA samples contain known sample indices and UMIs and thus resembles sc-RNA-seq read formats. Do you have a recommendation on how to use Salmon and / or Al…
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Thanks for your work! Excellent!
I have a question about the "Integration of expression data and binding matrices" section, related to figure 3A.
1. This should integrate multiple datasets, incl…
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Hi,
After reading the tutorials carefully, I still feel confused how to prepare the input data. In most cases, users want to get cell-type fractions from tumor bulk RNA-seq data using the 10X data…
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Hey,
I have a question: my matrix in the "calculate_reaction_score", considering bulk RNA data, must be normalized? In which way? TMM, TMP,log2? I don't see any directive.
Thank you
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I am currently working on a tumor bulk RNA-seq data set in order to identify somatic mutations. I have the BAM file for the tumor data set but I do not have the BAM file for a normal sample. Is there …
ghost updated
2 years ago
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Hi,
I would like to learn to run BayesPrism on some bulk RNA seq data as part of a research project. However, whenever I try to open the tutorial_deconvolution.html and tutorial_embedding_learning…
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Hi, I would like to try this approach on some dog data (B-cells Bulk RNA-seq) before/after treatment. What would be an example pair of input files for this? Thanks.
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Predict how many samples you need for your next RNA seq experiment using available data for estimation of variance in genes for different tumortypes
User controls 1. gene, 2. tumorType, 3. isCellLi…