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I tried the program on PacBio hifi reads, which were for amplicon sequencing of some plant DNA samples, barcoded on both ends. The reason is that LIMA generated very simple report missing a lot of inf…
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Hi,
I tried running lotus2 but got the following error:
This is sdm (simple demultiplexer) 2.17 beta.
Checking for switched pairs.
Run with 12 cores.At lotus2/SRR13436190_1.fastq:
Undecided f…
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Good afternoon! I recently found your publication on MosaicHunter and would really love to use it. I was wondering, whether it would be possible to use MosaicHunter on total RNAseq data (TruSeq Stran…
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I run delly as follows:
delly_v1.1.5_linux_x86_64bit call -g GCF_000001735.4_TAIR10.1_genomic.fna SRR21814509.sorted.marked.bam > delly.vcf
but I encountered a warning!
Warning: Sample has a …
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Before we start, let us talk about one experimental design as an example to highlight what metadata we might need to annotate the files of this experiment . We have a sequencing experiment to test RNA…
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Our objective is to test TCR Ab Oligos to define starting cell type, and also to Test the ability to pool samples with TCR Ab Oligos.
We are using 10X for single cell analysis and illumina for s…
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I ran jellyfish count on ccs reads produced from PacBio HiFi sequencing as follows:
```
jellyfish count -C -m 31 -s 1000 -t 10 -o marten.pb.jf m64047_240219_192555.ccs.fasta
```
And subsequent…
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Hi SingleM team! I was wondering is it possible to drag out certian reads or know which reads are based on the taxonomy classification of SingleM? I am current consider using a smaller group of reads …
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Some papers use STAR with parameters TK .
But i cannt find the paremeter, can you tell me the function of "TK"
Thanks!
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Dear Kristoffer,
Thanks for developing and maintaining this tool.
I just want to ask a quick question on what to set as "readLength" on the importIsoformExpression function for Stringtie outputs f…